标记染料的光谱学性质
|
标记染料
|
吸收最大波长（nm）
|
摩尔消光系数（ε）
|
荧光发射最大波长（nm）
|
|
Acridine
|
362
|
11,000
|
非荧光物质
|
|
Alexa Fluor 488
|
495
|
71,000
|
519
|
|
Alexa Fluor 532
|
531
|
81,000
|
554
|
|
Alexa Fluor 594
|
590
|
73,000
|
617
|
|
AMCA(7-Amino-4-methylcoumarin)
|
353
|
19,000
|
442
|
|
Bodipy R6G
|
528
|
73,000
|
550
|
|
Bodipy FL
|
505
|
80,000
|
513
|
|
Bodily 493/503
|
500
|
89,000
|
506
|
|
Bodily 564/570
|
563
|
142,000
|
569
|
|
Bodily 581/591
|
581
|
136,000
|
591
|
|
Bodily 630/650
|
625
|
101,000
|
640
|
|
Bodily 650/665
|
646
|
102,000
|
660
|
|
CY3
|
550
|
150,000
|
570
|
|
CY5
|
643
|
250,000
|
667
|
|
DABCYL
|
453
|
32,000
|
非荧光物质
|
|
DEA(Diethylaminocoumarin)
|
443
|
57,900
|
472
|
|
D2-PA
|
750
|
170,000
|
770
|
|
D3-PA
|
685
|
224,000
|
706
|
|
D4-PA
|
650
|
203,000
|
670
|
|
ECLIPSE
|
522
|
--
|
非荧光物质
|
|
FITC
|
492
|
78,000
|
520
|
|
5-FAM
|
495
|
74,000
|
520
|
|
6-FAM
|
494
|
83,000
|
518
|
|
6-FAM(ABI)
|
494
|
74,850
|
518
|
|
HEX
|
533
|
98,000
|
559
|
|
HEX(ABI)
|
535
|
95,698
|
553
|
|
JOE
|
520
|
75,000
|
548
|
|
NAP(Naphthofluorescein)
|
603
|
49,200
|
670
|
|
NBD
|
466
|
22,000
|
534
|
|
Oregon Green 488
|
492
|
88,000
|
517
|
|
Pyrene
|
345
|
44,000
|
378
|
|
Rhodamine Green
|
504
|
78,000
|
533
|
|
ROX(X-rhodamine)
|
587
|
82,000
|
607
|
|
Texas red(Sulforhodamine 101)
|
568
|
--
|
590～630
|
|
TAMRA(Tetramethylrhodamine)
|
560
|
89,000
|
582
|
|
TET
|
521
|
99,000
|
536
|
|
TET(ABI)
|
521
|
85,553
|
538
|
|
Texas red-X
|
583
|
107,000
|
603
|